Resep Selapis Keratinosit

Reagen	Larutan alikuot	Konsentrasi tujuan	50 ml	100 ml
DMEM/F12 3:1	1x	1x	Add 50 ml	Add 100ml
FBS ( di Inaktivasi)	1x	5% total	2,5 ml	5 ml
Hidrokortison	50 ug/ml	10 ug/ml	10 ml	20 ml
ITS	(1 mg/ml)	10 ug/ ml	0,5 ml	1 ml
Amphotericin B	250 ug/ml	2,5 ug/ml	0,5 ml	1 ml
Pen-strep	5000 U pen-5 mg strep /ml	100 U P- 100 ug/ ml	1 ml	2 ml
L-glutamin	200mM	2 mM	0,5 ml	1 ml
EGF	50 ug/ml	10 ng/ml	10 ul	20 ul
KGF/FGF-7	500 ng/ml	1 ng/ml	100 ul	200 ul
BPE	1 mg/ml	60 ug/ml	3 ml