Senin, 11 Oktober 2010

Resep Selapis Keratinosit

Reagen

Larutan alikuot

Konsentrasi tujuan

50 ml

100 ml

DMEM/F12 3:1

1x

1x

Add 50 ml

Add 100ml

FBS ( di Inaktivasi)

1x

5% total

2,5 ml

5 ml

Hidrokortison

50 ug/ml

10 ug/ml

10 ml

20 ml

ITS

(1 mg/ml)

10 ug/ ml

0,5 ml

1 ml

Amphotericin B

250 ug/ml

2,5 ug/ml

0,5 ml

1 ml

Pen-strep

5000 U pen-5 mg strep /ml

100 U P- 100 ug/ ml

1 ml

2 ml

L-glutamin

200mM

2 mM

0,5 ml

1 ml

EGF

50 ug/ml

10 ng/ml

10 ul

20 ul

KGF/FGF-7

500 ng/ml

1 ng/ml

100 ul

200 ul

BPE

1 mg/ml

60 ug/ml

3 ml

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